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Cervical screening, HPV and the abnormal result

Gynaecological oncology

A cancer that screening prevents: HPV testing is replacing cytology, colposcopy finds the lesion, and vaccination is closing the loop.

The programme

  • Persistent infection with high-risk HPV (16 and 18 in about 70 percent of cancers) causes cervical intraepithelial neoplasia at the transformation zone over years; screening finds it before invasion.
  • Canadian provinces are moving from cytology every 3 years (from age 21 or 25) to primary HPV testing every 5 years (from 25 or 30), with self-sampling in several programmes. Intervals and start ages differ by province; check the programme, not a memory.
  • HPV vaccination (9-valent) is routine in school programmes and offered to adults to age 45; it prevents the infections that cause most cervical, anal, oropharyngeal and vulvar cancers.
  • Screening stops around 65 to 70 with an adequate negative history; it is not needed after a hysterectomy for benign disease with the cervix removed.

The abnormal result

Low-grade squamous intraepithelial lesion with HPV effect: koilocytes beside normal squamous cells.
  • Colposcopy: acetic acid turns dysplastic epithelium white; Lugol's iodine is not taken up by it. Biopsy the worst area. CIN 1 is watched; CIN 2 or 3 is treated by LEEP (loop excision) or ablation, with a small increase in preterm birth risk after excision.
  • In pregnancy, screening is done if due and colposcopy is safe; treatment is deferred unless invasion is suspected.
ResultNext step (typical programme)
HPV negativeRoutine recall
HPV positive, cytology normalRepeat in 12 months; colposcopy if persistent
HPV 16 or 18 positiveColposcopy
ASC-US, LSILHPV triage or repeat cytology; colposcopy by programme rules
ASC-H, HSIL, AGCColposcopy (and endocervical or endometrial sampling for AGC)

Watch it done

Cervical screening sample (OSCE guide)Geeky Medics on YouTube

Consent, positioning, speculum, brush sampling and the liquid-based cytology vial.

Speculum examination (OSCE guide)Geeky Medics on YouTube

On the modelCervix

Frameworks and sources

  • Canadian Task Force on Preventive Health Care / provincial programmes: Cervical cancer screening recommendations and provincial HPV-based screening programmes (2025). Intervals and start ages differ by province and are changing as HPV primary screening rolls out; the module teaches the principles and tells the learner to check their programme.

Content reviewed 2026-09. Teaching material for students, not clinical decision support; check the current edition of the guideline your unit uses.